sellekchem Because expression of dksA is required for S. flexneri virulence, and growth of Shigella in the intracellular environment may induce a stress response, we also mea sured invasion and plaque formation by the gluQ rs mutant. However, no significant differences were Inhibitors,Modulators,Libraries noted, suggesting that GluQ RS is not essen tial for invasion or intracellular growth of S. flexneri. Discussion Conserved dksA gluQ rs genomic organization in gammaproteobacteria GluQ RS, a paralog of GluRS synthetase, is involved in the formation of GluQ, the nucleoside located at the wob ble position of tRNAAsp in bacteria. The protein is present in Firmicutes, Actinobacteria, Cyanobacteria, Alphapro teobacteria, Betaproteobacteria, Gammaproteobacteria and Deltaproteobacteria.
From the phylogenetic analysis we distinguished the three subgroups described previously based on the HIGH motif that is present in the class I aminoacyl tRNA synthetases. As was described Inhibitors,Modulators,Libraries previously, all GluQ RS enzymes are charac terized by the replacement of a threonine in GluRS enzymes, Inhibitors,Modulators,Libraries which is involved in the recognition of the amino acid and the terminal adenosine of the tRNAGlu by isoleu cine, leucine or valine at that position. This substitution is also conserved in all enzymes analyzed here, including those from the Firmicutes group. The gluQ rs gene is widely distributed in the bacterial do main. however, its genome organization is variable. We observed that only in members of the gammaproteobacteria, namely Aeromonadales, Alteromonadales, Pseudomonadaceae, Stringent response and tRNA modification Our bioinformatic analysis shows the presence of a tran scriptional terminator and lack of a gluQ rs specific promoter.
This is consistent with our results, in which we did not detect any activity from promoters other than those upstream of the dksA gene. This unusual arrangement suggests that gluQ rs expression is dependent on dksA regulated conditions. Because Inhibitors,Modulators,Libraries DksA is a key member of the stringent response in bacteria and regulates a number of Inhibitors,Modulators,Libraries processes in the cell, including its own expression, the data suggest that there is coordinate regulation of tRNA modification and other DksA targets. Although we could not detect any promoter activity spe cific for gluQ rs in the growth conditions tested, we cannot discount the possibility that the gene is specifically regulated under some other conditions. The regulon database indicates that the E. coli gluQ Tubacin supplier rs gene has a recognition site for the ��24 subunit of RNA polymerase. From our analysis, this sequence is iden tical to S. flexneri, but there is no experimental evidence of this recognition. Interestingly, when the gluQ rs gene was deleted in S. flexneri, the mutant showed impaired growth in the presence of osmolytes.