The test organisms were Rhizopus oryzae (MTCC 262), Chrysosporium tropicum (MTCC) and Aspergillus niger Selleck Compound Library (MTCC 281). Cultures of test organisms were maintained on potato dextrose agar slants and were subcultured in petri dishes prior to testing. The readymade potato dextrose agar medium (39 g) was suspended in distilled water (1000 ml) and heated to boiling until it dissolved completely. The medium and the petri dishes were autoclaved at a pressure of 15 ib/inch for 20 min.

Stock solutions were prepared by dissolving compound in DMSO and different concentrations were prepared (30 μg/ml). Agar cup bioassay was employed for testing antifungal activity of plant extract following the standard procedure. 14 The Crizotinib clinical trial medium was poured into petri dishes under aseptic conditions in a laminar flow chamber. When the medium in the plates solidified, 0.5 ml of 24 h old culture of test organism was inoculated. After inoculation, cups were scooped out with 6 mm sterile cork borer and the lids of the dishes were replaced. To each cup different concentration of test solutions (30, 100 μg) were added. Controls were maintained with DMSO using sample Clotrimazole. The treated and the control samples were kept at RT for 24–96 h

and inhibition zones were measured and diameter was calculated. Clotrimazole is taken as standard reference agent. (6a) 5-(phenyl)-4-methyl-3yl-(Imidazolidin-1ylmethyl, 2-ylidene nitro imine) isoxazole IR: νmax: 3310, 1580, 1590, 1410, 1297 cm−1, 1H NMR: δ 5.3 (s, 2H, –CH2–N–), 2.3 (s, 3H, isoxazole–CH3), 2.1 (brs, 1H, –NH), 2.8–3.1 (m, 4H, CH2), 7.4–7.55 (m, 3H, Ar.H), 7.7–7.8 (m, 2H, Ar.H), EI mass (m/z) 301 (M+), 247, 216. (6b) 5-(4-chlorophenyl)4- methyl-3yl-(Imidazolidin-1ylmethyl, 2-ylidene nitro imine) isoxazole IR: νmax: 3310, 2998, 1580 cm−1, 1H NMR: δ 5.5 (s, 2H, –CH2–N), 2.3 (s, 3H, isoxazole–CH3), 2.1 (brs, 1H, -NH), 2.9–3.2 (m, 4H), 7.4 (d, 2H, Ar.H, J = 8.0 Hz),7.65 (d, 2H, Ar.H = 8.2 Hz), EI mass (m/z) 335 (M+), 262, 247, 111. (6c) 5-(4-bromophenyl)-4-methyl-3yl-(Imidazolidin-1yl methyl, 2-ylidene nitro imine) isoxazole second IR: νmax: 3310, 1580, 1415, 1297 cm−1, 1H NMR: δ

4.6 (s, 2H, –CH2N–), 2.4 (s, 3H, isoxazole–CH3), 2.2 (brs, 1H, –NH), 2.7–3.1 (m, 4H), 7.5 (dd, J = 7.9 and 2.5 Hz, 2H, Ar.H), 7.8 (dd, J = 8.1 and 2.4 Hz 2H, Ar.H), EI mass (m/z) 379 (M+), 262, 225. (6d) 5-(4-flourophenyl)-4-methyl-3yl-(Imidazolidin-1ylmethyl, 2-ylidenenitroImine)isoxazole. IR: νmax: 3411, 1586, 1417, 1296 cm−1, 1H NMR: δ 5.5 (s, 2H, –CH2–N–), 2.3 (s, 3H, isoxazole–CH3), 2.10 (brs, 1H, –NH), 2.55–2.8 (m, 4H), 7.15 (m, 2H, Ar.H), J = 8.5 Hz, 7.75 (m, 2H, Ar.H), EI mass (m/z) 319 (M+), 270, 245. (6e) 5-(4-methyl phenyl)-4-methyl-3yl-(Imidazolidin-1ylmethyl, 2-ylidene nitro imine) isoxazole IR: νmax: 3406, 1555, 1410 cm−1, NMR: δ 2.4 (s, 3H, –ArCH3), 5.4 (s, 2H, –CH2–N), 2.2 (s, 3H, isoxazole–CH3), 2.1 (brs, 1H, –NH), 2.6–3.1 (m, 4H), 7.3 (d, 2H, Ar.H, J = 7.5 Hz), 7.7 (d, 2H, Ar.H = 7.