In the field of medicine, TASKI Protasan (TP) and TASKI Combatan (TC) are in use as effective compounds against bacteria, virus and fungi including human immunodeficiency

and hepatitis virus.6 While wards and corridors of hospital; research and development institutions have to be disinfected daily to keep up hygiene a wide spectrum of microorganisms and accurate dosing of medical disinfectants is required. Hence, the effectiveness of TP and TC on B. mori and NPV were examined to corroborate the use of Benzalkonium Chloride (BC), one of the components of TP and TC, as a common preservative in ophthalmic solution 7 and disinfectants in healthcare centers and food processing industries. 8 JAK inhibitor The silkworm, Bombyx mori strain NB4D2 and nucleopolyhedrovirus derived from grasserie diseased larvae were used. Commercially available TP and TC were procured from Qualigens Fine Chemicals, Mumbai.9 The compositions are TP – benzalkonium chloride (11.05% w/w) and nonionic surfactants; TC OSI-744 – benzalkonium chloride (10% w/w), polymeric biguanide hydrochloride (12% w/w), formaldehyde (15% w/w) and ethane dialdehyde (30% w/w). After standardizing the dosage through base experiments 0.1, 0.5 and 1.0% of TC and TP was considered for further studies. Accordingly, healthy silkworm

larvae in three replications with 50 larvae each in all the treatments including control were maintained. Mulberry leaves treated with 0.1, 0.5 and 1.0% of TP and TC for 5 min, which dried under shade were fed to fifth instar newly exuviated larvae and continued until spinning at 48 h intervals as one of the feeds per day. A control batch was fed with mulberry leaves immersed in distilled water. The quantum of leaves fed to all the batches of silkworm larvae was uniform. Haemolymph drawn from the larvae into a tube containing phenylthiourea was centrifuged at 3600 rpm for 5 min.10 and 11 The sediment containing polyhedral inclusion bodies (PIB’s) washed twice in 0.85 N NaCl and centrifuged at 3000 rpm.

The sediment suspended in 0.2 M sodium phosphate buffer (pH 7.6) was centrifuged at 3600 rpm for 20 min. Finally, the suspension was mixed with an equal volume of glycerol and centrifuged at 10,800 rpm for 30 min. The polyhedral bodies were re-suspended in distilled water Idoxuridine and strength of the stock was determined using haemocytometer as follows, Formula: concentration = X × 100 (where, X is the number of PIB’s), For example: X = A + B + C + D + E Total PIB’s X = 49 + 60 `+ 67 + 51 + 65; X = 292. Therefore, the concentration of primary stock was 292 × 100 = 2,92,000 (2.92 × 105 PIB’s/μl). (Standards: LC25 = 89 PIB’s/μl, LC50 = 266 PIB’s/μl, LC75 = 795 PIB’s/μl, LC95 = 3864 PIB’s/μl). i.eLC50 =266  2.92 × 105=91.09×105=9.1×105=9.1μlofPIB’s LC50 = 9.1 μl of PIB’s suspension to 990.9 μl of distilled water.