Somaclonal variation can provide an alternative for improvement
of existing genotypes. Six hundred and twenty-seven somaclones were developed from sugarcane genotype S97US297, and protocols for callogenesis and organogenesis were developed using Murashige and Skoog medium. Two types of explants, leaf and pith, and two auxins, 2,4-dichlorophenoxy acetic acid and indole-3-acetic beta-catenin mutation acid, were tested to optimize callogenesis for root establishment. Leaves as explants with 3.0 mg/L 2,4-dichlorophenoxy acetic acid gave the best results, both for callus induction and proliferation. Half-strength Murashige and Skoog medium with 1.5 mg/L indole-3-butyric acid proved to be the best for rooting. Red rot-resistant somaclones of the R(2) generation along with the parent were assessed for genetic variability at the molecular level using RAPD and SSR markers. Polymorphism based on RAPD and SSR was 32 and 67%, respectively. Polymorphic information content ranged from 0.06-0.45 for RAPD and 0.06-0.47 for SSR. We conclude that somaclonal variation of sugarcane
varieties is sufficient to allow selection.”
“The behavior of hydrogen molecules adsorbed onto the nanospace of porous materials is of great interest but is poorly understood. Here we show direct visualization of deuterium molecules adsorbed on isoreticular metal organic frameworks (IRMOF-1) at 77 K using reverse Monte Carlo simulation based on first principle molecular dynamics simulation and neutron diffraction. Results show that the two types of adsorption sites around a ZnO(4) cluster are almost fully occupied by the deuterium molecules but that other sites are not fully occupied. Moreover an interesting information Cl-amidine clinical trial about the aggregation state of
deuterium molecules was directly obtained from the deuterium-deuterium partial pair distribution function. Namely, the average distance of deuterium molecules adsorbed onto IRMOF-1 is slightly longer than that in a solid state but much shorter than that in the corresponding gas state. (C) 2010 American Institute of Physics. [doi:10.1063/1.3490625]“
“Background The role of genital mycoplasmas, particularly Ureaplasma urealyticum, in the etiology of infertility has been very controversial. An attempt has been made YM155 Apoptosis inhibitor to explore the role of mycoplasma in unexplained infertility.
Methods The study was conducted on 100 women, divided into two groups: 50 cases with unexplained infertility (study group) and 50 cases with confirmed fertility (control group). Three cervical swabs were collected from each case and sent for bacteriological examination for mycoplasmas. Statistical analysis was performed with chi-square test.
Results Mycoplasmas were isolated from 19 cases (38%) in study group and six cases (12%) in the control group. Out of these, 16 cases (32%) and 4 cases (8%) in the study and control group respectively were positive for U. urealyticum and the difference was statistically highly significant (P < 0.02).