methylohalidivorans cultures

methylohalidivorans cultures sellectchem and Evelyne-Marie Brambilla for DNA extraction and quality control (both at the DSMZ). The work conducted by the U.S. Department of Energy Joint Genome Institute was supported by the Office of Science of the U.S. Department of Energy under contract No. DE-AC02-05CH11231; AL was supported by Russian Ministry of Science Mega-grant no.11.G34.31.0068; SJ O’Brien Principal Investigator. The work conducted by the members of the Roseobacter consortium was supported by the German Research Foundation (DFG) Transregio-SFB 51 with PhD stipends for NB and AF. We also thank the European Commission which supported phenotyping via the Microme project 222886 within the Framework 7 program.
Strain G2T (= CSUR P206 = DSM 26159) is the type strain of B. massiliogorillae sp. nov.

This bacterium is a Gram-variable, facultatively anaerobic, indole-negative bacillus having rounded-ends. It was isolated from the stool sample of Gorilla gorilla gorilla as part of a ��culturomics�� study aiming at cultivating bacterial species within gorilla feces. The genus Bacillus (Cohn 1872) was created about 140 years ago [1]. To date this genus, comprised mostly of Gram-positive, motile, and spore-forming bacteria, includes 276 species with validly published names [2]. Members of the genus Bacillus are ubiquitous bacteria isolated from various environments including soil, fresh and sea water, food, and occasionally from humans and animals in which they are either pathogens, such as B. anthracis (the causative agent of anthrax) [3] and B.

cereus (associated mainly with food poisoning) [4], or saprophytes [5]. Bacillus species may also rarely be involved in a variety of human infections, including pneumonia, bacteremia, meningitis, endocarditis, endophthalmitis, osteomyelitis and skin/soft tissue infection [5]. However, in great apes, few data are available about the presence of the genus Bacillus. Recent reports have described the isolation of atypical B. anthracis (B. anthracis-like bacteria) in wild chimpanzees and gorillas from Africa [6-8]. Here we present a summary classification and a set of features for B. massiliogorillae sp. nov. strain G2T together with the description of the complete genome sequence and annotation. These characteristics support the circumscription of the species B. massiliogorillae [9].

Classification and features In July 2011, a fecal sample was collected from a wild western lowland gorilla near Messok, a village in the south-eastern part of the DJA FAUNAL Park (Cameroon). The collection of the stool Cilengitide sample was approved by the Ministry of Scientific Research and Innovation of Cameroon. No experimentation was conducted on this gorilla. The fecal specimen was preserved at -80��C after collection and sent to Marseille. Strain G2T (Table 1) was isolated in January 2012 by cultivation on Brucella agar medium (Oxoid, Dardilly, France).

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