The sample size of this study was calculated using odds ratio (OR

The sample size of this study was calculated using odds ratio (OR), with significance α level of 0.05, power of 0.80, and a sampling ratio of 1:1 between those exposed and unexposed to the predictor. An OR in the source population equal to 2.5 and an expected frequency of stature equal to 0.08 among the unexposed population were assumed. Due to high costs and difficulty of obtaining blood samples, it was considered that a sample of approximately 50% of the survey population was sufficient to test the hypothesis

of the study. The maximum acceptable beta error was 0.20. Subjects were selected for the study according to their nutritional status. Prior to the commencement of the study, participants underwent clinical examinations, as well as blood, urine, and parasitological tests. Individuals diagnosed with genetic and neurological syndromes, dementia, or SCH 900776 price cardiovascular, respiratory, or metabolic disorders were excluded from the www.selleckchem.com/screening/gpcr-library.html study, as were those using anti-inflammatory medications and those with

physical limitations. Subjects presenting infectious or parasitic diseases were treated according to normal protocols and subsequently included in the study. Socioeconomic and environmental data were obtained from parents or legal guardians by application of a specific questionnaire. The weight of each participant (wearing light clothes and without shoes) was obtained by single measurement using a Country Technologies model SD-150 platform scale (Gays Mills, WI, USA) with a capacity of 150 kg and an accuracy of 100 g. Stature was assessed using an AlturExata (TBW, São Paulo, Brazil) portable stadiometer with a precision of 0.1 cm. BMI values were calculated as weight (kg)/height squared (m2). For the determination of waist circumference, subjects were placed in a standing position with the abdomen and arms relaxed alongside the body, and a flexible measuring tape (1 mm accuracy) was held horizontally Carnitine palmitoyltransferase II at the midpoint between the bottom edge of the last rib and the iliac crest. The waist circumference deciles were calculated for the entire population and

compared to the values of stature (cm). Blood pressure was measured using a standard clinical sphygmomanometer. Subjects were seated and allowed to rest for 10 min, following by three pressure measurements conducted at 5 min intervals. SAP was determined at the onset of the first Korotkoff sound, while DAP was determined after the disappearance of the fifth Korotkoff sound.18 Fasting plasma glucose concentrations were determined by a Beckman Coulter UniCell DXI 800 (CA, USA) spectrophotometer, while specific insulin (without C peptide) levels were assessed using an enzyme assay and a Medcorp Advia 2400/Kovalent (RN, Brazil) analyzer. Serum levels of total cholesterol, LDL-C, HDL-C, and triglycerides were measured using an Advia 2400/Kovalent analyzer.

Leave a Reply

Your email address will not be published. Required fields are marked *

*

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>