The rats were anesthetized with inhalation of 2% normally isoflurane. For all rats, the following sequences were acquired in the transverse plane, with a slice thickness of 2 mm and an inter-slice gap of 0.2 mm in the following order: 1. T2-weighted fast spin echo imaging (T2WI) with fat saturation and a repetition/echo time (TR/TE) of 3860/106 ms, a turbo factor of 19, a field of view (FOV) of 140 �� 70 mm, and an acquisition matrix of 256 �� 256. Three signals were acquired, in a scan time of 1 min 25 sec. 2. Diffusion weighted imaging (DWI) with a 2-dimensional (2D), spin echo, echo-planar imaging sequence. We used a TR/TE of 1700/83 ms, a FOV of 140 �� 82 mm, and an acquisition matrix of 192 �� 91 (in-plane resolution: 0.7 �� 0.9 mm).

For the DWI, six signals were acquired, including repeated measurements for 10 different b values (0, 50, 100, 150, 200, 250, 300, 500, 750, and 1000 s/mm2) in three directions (x, y, and z) and averaged for the calculation of the isotropic apparent diffusion coefficient (ADC) value. A parallel imaging technique was applied to reduce susceptibility artifacts and examination times. The total examination time was 4 min 51 s. 3. Dynamic contrast enhanced MRI (DCE-MRI) using a fat saturated 3D T1-weighted gradient echo sequence (volumetric interpolated breath-hold examination, VIBE). The following parameters were used: a TR/TE of 7.02/2.69 ms, a FOV of 81.3 �� 130 mm, parallel imaging with an acceleration factor of two and a matrix of 154 �� 192 (in-plane resolution: 0.5 �� 0.7 mm). In total, 80 measurements were acquired, each lasting 3.

7 sec, leading to a total scan time of 4 min 58 sec. A bolus of 0.04 mmol/kg gadoterate meglumine (Dotarem?, Guerbet, France), prepared with a gadolinium concentration of 0.5 mmol/ml was injected i.v. after the 20th measurement. 4. Dynamic susceptibility contrast MRI (DSC-MRI) with a T2*- weighted echo-planar imaging sequence with the following parameters: a TR/TE of 2000/46 ms, a FOV of 140��70 mm, parallel imaging with an acceleration factor of two and a matrix of 128��128; (in-plane resolution: 1.1��0.5 mm). In total, 80 measurements were acquired, each lasting 2 sec, leading to a scan time of 2 min 46 Cilengitide sec. An i.v. bolus of 0.3 mmol/kg Dotarem ? was given after the 20th measurement. 5. Contrast-enhanced fat saturated T1-weighted fast spin echo imaging (CE-T1WI) immediately after the DSC-MRI sequence, with the following parameters: a TR/TE of 535/9.2 ms, a turbo factor of seven, a FOV of 140 �� 70 mm, and an acquisition matrix of 256 �� 256 (in-plane resolution: 0.5 �� 0.3 mm). Four signals were acquired, in a scan time of 1 min 24 sec. The total examination time for the whole MRI protocol was 15 min 35 s.