The detection of QD-labelled infected erythrocytes by flow cytometry was sensitive enough to monitor chloroquine anti-malarial toxicity with a drug incubation period
as short as 24 h (EC(50) = 113nM). A comparison of our assay with another widely used anti-malarial drug screening assay, the pLDH assay, showed that PCQD-based assay had 50% improved sensitivity in detecting drug efficacy within a parasite life cycle. An excellent Z-factor of 0.8 shows that the QD assay is suitable for high-throughput screening.
Conclusions: This new assay can offer a rapid and robust platform to screen novel selleck chemical classes of anti-malarial drugs.”
“Glycidyl methacrylate functionalized acrylonitrile-butadiene-styrene (ABS-g-GMA) particles were prepared and used to toughen polylactide (PLA). The characteristic absorption at 1728 cm(-1) of the Fourier transform infrared spectra indicated that glycidyl methacrylate (GMA) was grafted onto the polybutadiene phase of acrylonitrile-butadiene-styrene (ABS). Chemical reactions analysis indicated that compatibilization and crosslinking reactions took place simultaneously between the epoxy groups of ABS-g-GMA and the end carboxyl or hydroxyl groups of PLA and that the increase of GMA selleck screening library content improved the reaction degree.
Scanning electron microscopy results showed that 1 wt % GMA was sufficient to satisfy the compatibilization and that ABS-g-GMA particles with 1 wt % GMA dispersed in PLA uniformly. A further increase of GMA content induced the agglomeration of ABS-g-GMA
particles because of crosslinking reactions. Dynamic mechanical analysis testing showed that the miscibility between PLA and ABS improved with the introduction of GMA onto ABS particles because of compatibilization reactions. The storage modulus decreased for the PLA blends with increasing GMA content. The decrease in the storage modulus was due to the www.selleckchem.com/products/lcl161.html chemical reactions in the PLA/ABS-g-GMA blends, which improved the viscosity and decreased the crystallization of PLA. A notched impact strength of 540 J/m was achieved for the PLA/ABS-g-GMA blend with 1 wt % GMA, which was 27 times than the impact strength of pure PLA, and a further increase in the GMA content in the ABS-g-GMA particles was not beneficial to the toughness improvement. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 122:2992-2999, 2011″
“PURPOSE: To determine the possible effect of corneal collagen crosslinking (CXL) with riboflavin and ultraviolet A (UVA) on intraocular pressure (IOP) measurements by Goldmann applanation tonometry (GAT).
SETTING: Institute of Vision and Optics, Faculty of Medicine, University of Crete, Heraklion, Crete, Greece.
DESIGN: Prospective case series.
METHODS: This noncomparative study measured IOP by GAT before CXL and 6 months and 12 months after CXL.
RESULTS: The study evaluated 55 eyes (55 patients).