The animals were intubated and ventilated with room air and isoflurane. Expiratory CO2 was monitored. Heparin, 5000 worldwide units was injected intravenously. Blood was collected, and the center was isolated using a thoracotomy. The heart was perfused in a Langendorff setup utilizing a combination of blood and Tyrodes solution. Atrioventricular block was made by smashing the AV nodal area. The left anterior descending coronary artery was opened over a distance of 5 mm, above the primary diagonal branch. A ligature was passedunderneath the LAD, and a cannula was introduced using a small cut into the LAD. The cannula was fixed by tying the ligature and was connected to another perfusion system via a miniature heat exchanger. The temperature of both perfusion areas was handled by split up heat exchangers in each perfusion branch. Infusion pumps were connected to the medial side branch of the LAD cannula and towards the aortic cannula for the administration of sotalol and/or flecainide. The absence of ST T segment changes indicated absence of local ischemia. Flecainide was uniquely infused in both vascular sleep, Metastasis depending on the preexisting inducibility of VF. Electrophysiology A rectangular grid of 11 electrodes was sutured over the border between the myocardium perfused by the LAD and the relaxation of the heart. The cyanotic line was identified prior to application of the electrode by a 30 s closure of the LAD. Correct positioning of the electrode was approved by creating a 5 min occlusion of the LAD and considering the line involving the area with and without electrophysiological signs of ischemia. After restoration of the flow of blood the guts was allowed to recover for at the very least 60 min before measurements were begun. Total recovery was described by the return of ST segment elevation to the isoelectric point MAPK cancer and a stable value of refractoriness in the LAD area. Unipolar cathodal stimulation was performed through one of many electrodes within the grid overlying the circumflex area. One to three stimulation positions were analyzed sequentially. The anode was placed in the aortic root. Premature beats were released after each and every practice of eight beats with coupling intervals ranging from the essential cycle length of 600 ms down to the refractory period. Control recordings were manufactured from a premature beat and a fundamental beat prior to the treatments. Regional unipolar electrograms were recorded against a reference electrode in the aortic root employing a data-acquisition system. Analysis of the electrograms was done offline employing a custom made analysis program. Local activation times were measured at the moment of the minimum dV/dt of the initial deflection, and regional repolarization times at the moment of the maximum dV/dt of the T wave. When determination of activation times was difficult because of fractionation of the indicators, Laplacian electrograms were constructed to aid in the detection of local activation.