RESULTS: Lipase production in submerged cultures of Yarrowia lipo

RESULTS: Lipase production in submerged cultures of Yarrowia lipolytica CECT 1240 (ATCC 18942) has been investigated. Significant lipolytic activity (over 700 U dm(-3)), mostly extracellular and membrane-bound, was obtained in shake flasks using medium supplemented with olive oil. The culture was carried out in air-lift Selleck CFTRinh 172 and stirred tank bench-scale bioreactors and the latter was selected. The influence of aeration and agitation rates was assessed in batch cultures, and agitation from 400-700 rpm and low aeration rates (i.e. 0.2 vvm) are recommended. Batch, fed-batch and continuous operation were investigated, and regular enzyme production (up to 600 U dm(-3)) was achieved with the latter.

CONCLUSION:

Lipase production by the selected strain was successfully carried out in shake flasks and bench-scale bioreactors.

After studying batch, fed-batch and continuous click here processes, continuous culture in a stirred tank bioreactor was found best in terms of regular enzyme production, exceptionally good operational stability and good fitting of the results to mathematical models. (C) 2009 Society of Chemical Industry”
“The optimization of the nutritional management of preterm infants has gained importance due to the increased survival of these vulnerable infants. In fact, the achievement of an adequate growth has been associated with a favourable neurodevelopment outcome. Nevertheless, preterm infants still develop severe nutrient deficiencies during the first few weeks of life so that they are frequently growth Quizartinib restricted upon discharge. In addition, preterm infants have been found to show an increased and aberrant adiposity at term-corrected age. The development of strategies immediately after birth has been found to produce excellent results in terms of growth without having any detrimental effect on body composition at term-corrected age. Continuing to monitor growth and body composition changes in relation to different nutrition interventions is essential as growth pattern and body composition appear to have a long term effect on health outcomes.”
“BACKGROUND:

The Fenton process is a popular advanced oxidation process (AOP) for treating textile wastewater. However, high consumption of chemical reagents and high production of sludge are typical problems when using this process and in addition, textile wastewater has wide-ranging characteristics. Therefore, dynamically regulating the Fenton process is critical to reducing operation costs and enhancing process performance. The artificial neural network (ANN) model has been adopted extensively to optimize wastewater treatment. This study presents a novel Fenton process control strategy using ANN models and oxygen reduction potential (ORP) monitoring to treat two synthetic textile wastewaters containing two common dyes.

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