Patients below 18 years old or patients unable or unwilling to SB203580 clinical trial give written informed consent were not included. Patients were routinely included after the primary PCI-procedure, the following morning after the admission. Clinical

information was collected from hospital records and questionnaires obtained at the time of inclusion. Blood samples were collected at median time of 24 h after symptoms and 18 h after the PCI procedure, between 08:00 and 10:00 a.m. the following morning, except those with infarction during the weekend, which were included the following monday morning. In order to standardize blood sampling, and also to avoid any influence of diurnal variations and food intake, all samples were taken after an overnight fast. Serum was prepared by centrifugation for 10 min at 2000g and samples were stored at −80 °C until analysed. Circulating levels of sgp130, sIL-6R and IL-6 were determined by commercial ELISA (R&D Systems, Abingdon, Oxon, UK) and CRP with kits from DRG Instruments (Marburg/Lahn, Germany). In our laboratory, the inter-assay coefficients of variation (CV) were 5.2%, 3.6%, 10.5% and <5%. Routine blood samples were analysed by use of commercial methods. Electrochemiluminescence technology for quantitative measurement was used for repeated measures of TnT (3rd PI3K inhibitor generation cTroponinT,

Elecsys 2010, Roche, Mannheim, Germany). The lower detection limit of the assay is 10 ng/L with a recommended diagnostic threshold Amobarbital of 30 ng/L. The inter-assay coefficient of variation was 7%. TnT levels were determined

in blood samples collected immediately after the acute PCI and in repetitive samples with 4 h between the two first samples and then following every 12 h until a rise and fall curve was observed. The level used in this study was the highest measured during hospital stay for each patient. NT-proBNP was measured in serum using Elecsys proBNP sandwich immunoassay on Elecsys 2010 (Roche Diagnostics, Indianapolis, USA). The inter-assay coefficient of variation was 7%. Left ventricular ejection fraction (LVEF) was measured by echocardiography a.m. Simpson or by visual judgement before hospital discharge or at clinical follow-up within 3 months after the myocardial infarction. In case of repeated examinations, an average value was used. LVEF obtained after discharge was only included if the patient was hemodynamically stable and without rehospitalisation between the index myocardial infarction and the examination. Continuous variables are presented as median values (25, 75 percentiles) and categorical variables as proportions. As most measured biomarkers were skewly distributed, non-parametric methods were used throughout.