Myoclonus in-the hindlimbs was easily elicited by stimulation. These doses were based on those shown to enhance locomotor function in rats for mCPP and to enhance motor activity in normal rats for DPAT and D 5 HTP. We didn’t make an effort to discover a doseresponse relationship because of the high mortality associated with some of those drugs in the SEV team. MOD and SEV subjects were habituated to the behavioral testing preoperatively purchase Decitabine and examined after drug administration for up to 12 months post-operatively. Weekly of drug testing started with behavioral testing following saline injection. A 2 day wash out period separated different drug administrations. The rats were tested with mCPP and D FEN at 4weeks post injury, andwith DPAT and mCPP alone and in combination at 6 months post injury. At 9 days postinjury, theywere testedwith R 5 HTP. Because of unexpected death from the precursor drug, the SEV group lost 3 rats making 9 for behavioral assessment at 1-2 weeks. Finally, the ratswere retestedwithmCPP and D FEN at 12 weeks post injury. A second number of MOD subjects was tested at 9 weeks only with L 5 HTP as a way to replicate to the positive without likely influence from previous drug exposure. Performance was videotaped where appropriate and mirrors were useful for observation of contralateral limbs. All tests were scored by trained observers who had interrater reliability scores more than 95%. All observers Immune system were unacquainted with the group identification of the animals. Open field locomotor check Hindlimb function was considered in a open field using the BBB locomotor rating scale. Subjects were observed for 2 min by two experienced observers and scored from 0 to 21. Subjects were trained for 3 min/day inside the Eco 3/6 Treadmill apparatus. The treadmill measures 33 T 20 T 20 H with a working surface that is 17 R 2. 375 T. To be able to enhance data collection for these reports, the treadmill was set to a slow rate of 6. 5 m/min. Locomotor function was assessed by counting the number of weight supported steps taken over a treadmill in 3 min and expressed as a percentage of total steps. Non weight backed Icotinib measures were those by which neither knee or hindquarters were elevated above the surface. Data are expressed as the big difference in %WS between drug and saline administration. Subsequent treadmill instruction, the presence of hindlimb tremors was dependant on observation through the behavioral tests and hindlimb palpation. Tremors were scored on a 4 point scale. Depletion of serotonin followed by administration of serotonergic agents, especially 5 HT1A agonists, is shown to generate several stereotypies referred to as the serotonin syndrome.