LC fingerprint with the multi wavelength mix technique we can begin a fingerprint of Dhge. isatidis, consider its inner quality, and in the same time, provide the research for its clinical use. Kong noted the anti-bacterial activity of EtOAc extract from Dtc. Its LC and isatidis fingerprint. But, this kind of fingerprint at single wavelength discovery Ganetespib chemical structure can’t fully reveal the real quality of R. isatidis due to the limit of the established method. In this study, LC fingerprints of fat-soluble Dhge. isatidis extracts were successfully conducted by the multi wavelength mix approach. The typical LC fingerprints of fat soluble Kiminas. isatidis extracts from 11 roots by numerous wavelength mixture process were first established, and the reference chromatogram was also made with 24 common peaks. The aim of this work was to offer a model of chromatogram mix at multiple detection wavelengths to scientifically study complex or the undeveloped materials. The technology also provided a platform for the future research. Our study will eventually Urogenital pelvic malignancy make us more rational in the development and utilization of R. isatidis. 2 Materials and 2. 1 Instruments The chromatographic system contains a HITACHI L 2300 column oven, a HITACHI L 2130 pump and a HITACHI L 2200 autosampler. The detector used was a HITACHI L 2455 DAD detector. Peak areas were built-in quickly utilizing the HITACHI D 2000 Elite workstation. Other apparatus included a DL 60D ultrasonic product, an SHZ N water circulation vacuum pump, a rotary evaporator, an AP 9901S oil free vacuum/pressure pump and a Milli Q water purification system. 2. 2 Materials and reagents Eleven amounts of dried roots of I. indigotica natural product library Fort obtained from different areas in China were researched, R. isatidis samples were ground in to powder of the homogenous 24 mesh before the test. Anthranilic acid, benzoic acid, syringic acid and salicylic acid were supplied by Dr Peng Du and Mei Fen Xie. Indigo and indirubin were obtained from the National Institute for Get a grip on of Biological and Pharmaceutical Products. Tryptanthrin was contributed by Professor Jian Li Liu. Ethyl acetate, methanol, phosphoric p, ultra-pure water purified with a Milli Q water purification system were found in the experiment for removal. 2. 3 Chromatographic condition The separation was performed on a YMC C18 column. The mobile phase was methanol and 0. 2% H3PO4 water alternative system, and the gradient elution profile was: 0 10 min A:B to, 10 35 min A:B, 35 55 min A:B to, 55 85 min A:B to, 85 110 min A:B. The flow rate was 1. 0 mL/min, diagnosis wavelengths: 230 and 310 nm, line temperature: 301C, treatment volume: 20 mL.