In experiments with multiplicities of infection of approximately 3, an increase in the polynuclear phenotype was verified both qualitatively (Fig. 1A) and quantitatively (Fig. 1B). These results are consistent with Salubrinal their data using laboratory strains and confirm that C. trachomatis infection blocks or slows cytokinesis in infected cells. Figure 1 Confirmation of the polynuclear phenotype in cells infected with different C. trachomatis strains. Panel A: Fluorescence micrograph

of C. trachomatis strain LGV-434 inclusion (anti-LPS, red) within a GFP-positive cell (green), showing three nuclei (blue). The scale bar indicates 10 microns. Panel B: The percentage of polynuclear cells 30 h after infection of HeLa cells

with different C. trachomatis at an MOI of 3. Strains D/UW3 and J(s)6686 are shown, along with mock-infected cells. Statistical significance is indicated with the asterisk above the individual treatment groups, as compared to www.selleckchem.com/products/Adrucil(Fluorouracil).html mock-transfected cells (Student’s t-test, p < 0.001). Similar levels of significance were observed in a Kruskall-Wallis test (not shown). Distribution of CT223p at the inclusion membrane varies in different C. trachomatis strains CT223p is localized {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| to the inclusion membrane in cells infected by C. trachomatis at time points after 8 hours post infection (p.i.). Consistent with our previous work [25], patches of CT223p protein are readily detectable at time points 12 h p.i. and later (Fig. 2A-D). The localization of CT223p is different in cells infected by representatives of different C. trachomatis serovars. In cells fixed at early and middle time points p.i., the Sinomenine labeling in cells infected by different serovars is similar and is manifested as dash-like or patchy localization of protein at the inclusion surface (Fig. 2A, C). At late time points however, a difference becomes apparent, as the labeling CT223p of

a serovar J isolate (Fig. 2D) becomes more diffuse than in isolates of serovar L2 (Fig. 2B) and serovar D (not shown). These differences in labeling are independent of cell type (either McCoy or HeLa) or fixative (paraformaldehyde or methanol). Figure 2 Expression of CT223 at different times post infection and differential reactivity with specific antibodies. DNA in all panels is labeled with DAPI (blue) and the bar in panel F represents 10 microns in each image. Cells were infected at an MOI of approximately 0.2 and fixed with 100% methanol prior to antibody labeling. Panels A-D: Fluorescent microscopy of McCoy cells infected with either strain LGV-434 (A, B) or J/UW36 (C, D). Cells were fixed at different times p.i. (A: 12 h, C: 18 h, B, D: 38 h). In panels A-D, cells were labeled with monoclonal anti-CT223p antibody (green) and anti-HSP60 (red). Note that labeling of CT223p is patchy in each strain at the early times points p.i. (A, C) but the labeling is distinct between strains at 38 h p.i. (B, D).