Given the results of a previous study showing that TA systems are ubiquitous in VRE (Moritz & Hergenrother, 2007), and the current survey showing that TA systems are also highly prevalent and transcribed in MRSA and PA, it appears that these problematic bacterial pathogens would indeed be susceptible to TA-based antibacterial strategies. Specifically, activation of MazEFSa should be considered for MRSA, and activation of RelEPa or HigBAPa appear to be attractive strategies against PA. This work was supported

Romidepsin in vitro by National Institutes of Health Grant 2R01-GM068385. J.J.W and E.M.H. were partially supported by a National Institutes of Health Cell and Molecular Biology Training grant T32 GM007283. E.M.D. was partially supported by the Center for Nano-CEMMS (NSF DMI-0328162) at the University of Illinois. We thank the bacterial laboratories at Carle Foundation Hospital (Urbana, IL),

Memorial Medical Center (Springfield, IL), and Delnor Community Hospital (Geneva, IL) for the MRSA isolates. We also thank Cubist Pharmaceuticals Inc. (Lexington, MA) and Carle Foundation Hospital (Urbana, IL) for the PA isolates. J.J.W. and E.M.H. contributed equally to this work. Fig. S1. Alignment of mazEFSa sequences. Fig. S2. Alignment of parDEPa sequences. Fig. S3. Alignment of relBEPa sequences. Fig. S4. Alignment of higBAPa sequences. Fig. S5. Alignment of mazEFSa upstream (a) and downstream (b) flanking sequences. Fig. S6. Alignment of parDEPa upstream (a) and downstream (b) flanking sequences. Fig. S7. Alignment of relBEPa selleck kinase inhibitor upstream (a) and downstream (b) flanking sequences. Fig. S8. Alignment of higBAPa upstream (a) and downstream (b) flanking sequences. Table S1. Presence of TA Systems in MRSA and Pseudomonas aeruginosa. Table S2. Flanking regions of TA Systems in MRSA Atorvastatin and Pseudomonas aeruginosa. Please note: Wiley-Blackwell is not responsible

for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article. “
“Phytophthora ramorum,Phytophthora alni, and Phytophthora kernoviae present significant threats to biosecurity. As zoosporic oomycetes, these plant pathogens may spread through natural waterways and irrigation systems. However, survival of these pathogens in aquatic systems in response to water quality is not well understood. In this study, we investigated their zoospore survival at pH 3–11 in a 10% Hoagland’s solution over a 14-day period. The results showed that all three pathogens were most stable at pH 7, although the populations declined overnight irrespective of pH. Extended survival of these species depended on the tolerance of pH of their germinants. Germinants of P. alni ssp. alni and P.