Short infusions of sodium nitroprusside were also administered through this catheter to provide more arteriolar and venous dilation, although no goal blood pressure was established. The 2nd study bolus was done within 10 15 min of beginning nitroprusside. Mean blood pressure and heart-rate measurements of the examinations without and with nitroprusside were compared using price AG-1478 two tailed matched Students t check using commercially available software. ? Significance level was set at P 0. 05. For picture acquisition the dogs were placed in dorsal recumbency in a V trough over a scientific 64 MDCT reader. A 20 G catheter was put in the right cephalic vein for injection of contrast medium in order to avoid ability artifacts due to dense undiluted contrast medium within the cranial vana cava. 13 15 A test bolus, also known as time bolus, of 5 ml iodinated comparison medium?? followed by 20 ml physiologic saline chaser, was shot at 2 ml/s at maximum pressure limits of 300 lb/in. 2 having a dual barrel power injector. ?? To observe contrast channel introduction and to determine time to peak of contrast enhancement recurring transverse scans were obtained in 1 s time periods at the level of the heart including the aortic root. Time for you to peak opacification in the ascending aorta was noted. The research contrast medium bolus was built to maximize coronary artery opacification throughout the scan. The contrast medium bolus amount was adjusted to the requirement to cover the whole scan length with adequate arterial opacification. Therefore, as a function of scan length divided by frequency, the length of the scan, was multiplied by the injection rate to offer the size. A 30 ml physiologic saline chaser was used to enhance the contrast medium through the machine while cleaning contrast medium out of right and pifithrin alpha the CrVC ventricle to avoid streak artifacts. 13 This triggered a total of 1. 5 2. 6 ml/kg distinction medium per dog. After the delay to peak enhancement determined from the test bolus series, the scan of the heart was started using helical scan style at 120 kV, EKG modulated mA, 350 ms gantry rotation time 40 mm alarm collimation, heart price modified variable pitch. Pictures were reconstructed to 0. 625 mm isotropic solution using a soft tissue reconstruction kernel. The length of the whole procedure including test bolus, individual setup and coronary scan was approximately 10 15 min per dog, the scan time for coronary angiography itself was determined by the cranial to caudal FOV, but was approximately 10 s. After having a short break allowing for washout of the contrast medium from the first study bolus, nitroprusside was shot and the study bolus and scan process was repeated for each dog.