Cells were transfected with GFP-vector. Transduction efficiency based on the fluorescent signalwas analyzed by FCM. (A) PANC-1 cells without transfection were used as the blank control; (B) Cells transfected with GFP-shRNA as random control; (C) Cells transfected with GLI1-shRNA as selleck bio experiment group. (TIF) Click here for additional data file.(793K, tif) Figure S3 Transduction efficiency of PANC-1 cells by lentivirus vector, phase contrast and GFP expression under a fluorescent microscope. Transduction efficiency of PANC-1 cells by GLI1 silencing vector. PANC-1 cells were transfeced with the GLI1-shRNA vector. The corresponding phase-contrast image(left panel), the GFP fluorescence (middle panel) and the merged image (right panel) are shown at a magnification of ��200.
GFP expression reveals high transduction efficiency, with more than 90% of cells being transduced. (TIF) Click here for additional data file.(4.7M, tif) Figure S4 Electropheretogram of sonicated chromatin solution in different conditions. Sonicated chromatin solution in different conditions were electrophoresed on 1.5% agarose gel containing ethidium bromied. DNA sizes appear smear at a range of 100 bp to 1 kb range in 80 W group. (TIF) Click here for additional data file.(270K, tif) Figure S5 The result of sequence analysis of CHIP products which amplified by RegIV primer-D. The result showed that the sequence amplified with RegIV primer-D is the same as that of RegIV gene promoter region containing GLI1-binding site 4. (TIF) Click here for additional data file.
(46K, tif) Figure S6 The result of sequence analysis of CHIP products which amplified by RegIV primer-E. The result showed that the sequence amplified with RegIV primer-E is the same as that of RegIV gene promoter region containing GLI1-binding site 4. (TIF) Click here for additional data file.(81K, tif) Figure S7 The result of sequence analysis of CHIP products which amplified by RegIV primer-F. The result showed that the sequence amplified with RegIV primer-F is the same as that of RegIV gene promoter region containing GLI1-binding site 4. (TIF) Click here for additional data file.(2.7M, tif) Footnotes Competing Interests: The authors have declared that no competing interests exist. Funding: This work was supported in part by National Natural Science Foundation of China (No. 81072065), Foundation for Shanghai Science and Technology Committee (No.
09JC1412200, No. 09410705400), and Doctoral Fund of Ministry of Education of China (No. 20090072110022). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of Brefeldin_A the manuscript.
Cystic fibrosis (CF; MIM# 219700) is one of the most common autosomal recessive disorders and occurs at a rate of 1 in 1600�C3000 Caucasians.