C. Column diagram analysis for the proliferation indexes (PI) calculated in three different groups. PI in siRNA group was significantly lower find more than that in blank control group and negative control group respectively. D. Column diagram analysis for the RGFP966 actual absorbance of three different groups, the mean actual absorbance of siRNA group was significantly lower than that of the blank control group and the negative control group, respectively. (*P < 0.05, compared with blank control group and negative control group respectively) Additionally, MTT assay was performed to test the effects of transfection with JMJD2A siRNA

on the proliferation of MDA-MB-231 cells treated in three different groups. As shown in Figure 2D, there was no significant difference (P > 0.05) in the average actual absorbance between blank control group (2.136 ± 0.135) and negative control group (2.089 ± 0.115). The average actual absorbance in siRNA group (1.711 ± 0.087) was significantly lower than that in blank control group (P < 0.05) and negative control group (P < 0.05), respectively. Absorbance represents cell proliferation in MTT assay. The MTT assay results consistented with FCM results. These data indicated that transfection with JMJD2A siRNA could significantly reduce the proliferation of MDA-MB-231 cells. Silencing JMJD2A gene suppressed MDA-MB-231 cell migration and invasion in vitro As

displayed in Figure 3, cell migration was significantly decreased in siRNA group than in blank control group (P < 0.05) and negative control group (P < 0.05), Syk inhibitor respectively. Cells in siRNA group showed significantly decreased invasiveness, compared with blank control group (Figure 4; P < 0.05) and negative control group (Figure 4; P < 0.05). These results demonstrated that transfection with JMJD2A siRNA could reduce the migration and invasion of MDA-MB-231 cells. Figure 3 Knock down of JMJD2A resulted in suppressing Rho tumor cell migration. A. Cells in blank control group transversed the Transwell membrane. B. Cells in negative control group. C. Cells in siRNA group. D. Column

diagram analysis for the number of MDA-MB-231 cells in migration assay. The number of siRNA group (67 ± 10.2) was decreased compared with that of blank control group (173 ± 17.7) and negative control group (168 ± 16.4), respectively. (*P < 0.05, compared with blank control group and negative control group respectively) (Note: ×200) Figure 4 Knock down of JMJD2A resulted in suppressing tumor cell invasion. A. Cells in blank control group transversed the Transwell membrane. B. Cells in negative control group. C. Cells in siRNA group. D. Column diagram analysis for the number of MDA-MB-231 cells in invasion assay. The number of siRNA group (175 ± 14.4) was decreased compared with that of blank control group (327 ± 20.8) and negative control group (311 ± 15.3), respectively. (*P < 0.