Blood glucose was measured using a glucose analyzer (ACCU-CHEK Active,

Roche, Shanghai, China). Serum insulin was assayed using rat ELIZA kits (Mercodia, Sweden). Glutathione peroxides’ activity was measured using the Paglia and Valentines method.13 Serum concentration of MDA were measured by the modified thiobarbituric acid method (spectrophotometry); the intra and inter-assay coefficients of variation were 5.5 and 5.9%, respectively.14 Statistical Analysis The data, expressed Inhibitors,research,lifescience,medical as mean±SD, were first examined for normality of distribution. As they were normally distributed, they were analyzed using the one-way analysis of variance (ANOVA), followed by the Duncan Multiple Range test for pairwise comparisons. A P≤0.05 was considered statistically significant. Results Weight of the animals in the diabetic group Proteasome inhibitor receiving vehicle (256.9±6.7 g) was significantly (P=0.0001) lower than that of the control group (294.5±15.2 g). However, there was no significant difference between the weight of the diabetic Inhibitors,research,lifescience,medical rats receiving vehicle and those Inhibitors,research,lifescience,medical of the diabetic rats receiving 200 mg/kg/day PSO (267.9±17.2 g), 600 mg/kg/day PSO (267.9±17.2 g), 200 mg/kg/day SBO (261.4±6.7 g),

or 600 mg/kg SBO (262.9±13.8 g). Blood glucose of the diabetic rats receiving vehicle was significantly higher than that of the control rats. However, there was no significant difference between the blood glucose of the animals in the diabetic group receiving PSO (200 or 600 mg/kg) or SBO (200 or 600 mg/kg) (figure 1). Figure 1 Concentrations (mean±SDM, n=8 each) of fasting blood glucose in the normal Inhibitors,research,lifescience,medical control group (N.C), type 2 diabetic control group receiving vehicle (DM2-C), and type Inhibitors,research,lifescience,medical 2 diabetic group receiving pomegranate seed oil at 200 mg/kg/day (PSO200) or 600 … Serum insulin of the diabetic rats receiving vehicle

was significantly lower than that of the controls (P=0.0001) (figure 2). Serum insulin of the diabetic rats treated with PSO (200 mg/kg) was significantly higher than that of the diabetic rats during treated with vehicle (P=0.013). Moreover, serum level of insulin in the diabetic rats treated with PSO (600 mg/kg/day) was significantly higher than that of the rats treated with identical doses of SBO (P=0.05) (figure 2). Figure 2 Fasting serum insulin concentrations (mean±SDM, n=8 each) of the normal control group (N.C), type 2 diabetic control group receiving vehicle (DM2-C), and type 2 diabetic group receiving pomegranate seed oil at 200 mg/kg/day (PSO200) or 600 mg/kg/day … Serum TG (P=0.001), total cholesterol (P=0.003), and LDL-C (P=0.05) of the diabetic control rats were significantly higher than those of the normal control groups (table 1). However, the serum HDL-C of the diabetic rats treated with vehicle was significantly lower than that of the normal control group (P=0.001).