As the silver Temozolomide cell line nanoparticles are confined in the interior of the polymers, their growth will be physically restricted by the meshes, so the size and size distribution can be effectively controlled. Figure 2 FTIR eFT508 manufacturer spectra of (a) RSD-NH 2 and (b) silver/RSD-NH 2 nanohybrid. Figure 3 Schematic description of silver ammonia. Figure 

4 shows the TEM images and the corresponding histograms of four samples prepared with four different initial AgNO3 concentrations. Upon increasing the initial AgNO3 concentrations from 0.017 to 0.17 g/l, the mean particle sizes increased from 1.76 to 65.77 nm, meanwhile the size distribution also increased. When the AgNO3 concentration is 0.225 g/l, some silver nanoparticles are more than 100 nm. The mean size of silver nanoparticles determined by DLS is consistent with the results by TEM images. Figure 4 TEM images and corresponding histograms of silver LEE011 purchase colloid nanoparticles [AgNO 3 ] = 0.017 g/l (a), 0.085 g/l (b), 0.17 g/l (c), 0.225 g/l (d). Figure  5 shows the UV-vis spectra of silver nanoparticles recorded at

different times during the preparation. At the beginning time, one characteristic peak at 298 nm is observed due to pure RSD-NH2[1]. As the stirring time increases, a new peak appears between 400 and 450 nm. This confirms the appearance of nanocrystallites of the silver particles in the solution; the shifting of peak positions with time also indicates the growing size of silver nanoparticles. Furthermore, the height of the absorption peaks of the silver nanoparticles increases and the full width at half maximum (FWHM) of the peaks decreases with time, which indicate the increasing amount and improved crystallinity of silver nanoparticles [16, 17]. Figure 5 UV-vis spectra of silver colloid nanoparticles at different time points. (a) 0 h. (b) 1 h. (c) 6 h. (d) 12 h. (e) 24 h. (f) 48 h. (g) 1 week. (h) 2 weeks. [AgNO3] = 0.17 g/l. The information given by TEM micrographs and UV-vis spectra indicates that the silver nanoparticles can be successfully synthesized through the reaction between AgNO3 and RSD-NH2. However, when the silver nanoparticle solution

was non-intrusively placed for more than 24 h, a shining silver mirror appeared on the inner wall of the glass container L-gulonolactone oxidase and the color of the solution changed to black. This is due to the apparent agglomeration and oxidation of silver nanoparticles in the solution. We prepared silver nanoparticles with 0.085 g/l AgNO3, and the precipitated silver powders in the silver colloid were centrifuged, washed with methanol, and dried in air for XRD measurement. The result is shown in Figure  6. It clearly shows the (111), (200), (220), and (311) planes of the silver nanoparticles. As shown in Table  1, the size of silver nanoparticles calculated by using Scherrer’s equation resulted in an average particle size of 26 nm. The mean size of silver nanoparticles calculated by Scherrer’s equation is consistent with the results by TEM images.