827, Table 2). Sleepiness also did not differ before the nap (P = 1), indicating equal sleep debt in the two conditions. There were also no differences in positive or negative affect (Positive and Negative Affect Scale) between

the two stimulation conditions (before nap, positive affect, P = 0.257; before nap, negative affect, P = 0.433; after nap, positive affect, P = 0.558; after nap, negative affect, P = 0.326; Table 2). Monitoring of activity (by ActiWatches) did not reveal any difference between the tSOS and sham conditions, confirming that sleep pressure before the nap was similiar between the conditions. The present study demonstrates that tSOS applied during non-REM sleep in an afternoon nap, in comparison with sham stimulation, enhanced subsequent declarative learning of pictures, word

pairs, and word lists, whereas training of a procedural finger sequence http://www.selleckchem.com/products/ve-822.html tapping skill remained unaffected. As expected, tSOS increased the depth of non-REM sleep by increasing SWS and, as a hallmark of SWS, SWA. Acutely, tSOS phase-locked spindle activity to the up-state of the induced slow oscillation. In combination, these findings corroborate and extend previous observations (Van Der Werf et al., 2009) pointing to a causative role of SWA in providing capacities for encoding of new information in the hippocampus-dependent memory system for the upcoming period of wakefulness. The application FK506 manufacturer of tSOS oscillating at 0.75 Hz proved to be effective in enhancing SWA and SWS. The effects of tSOS are known to be state-dependent (Steriade et al., 1993; Kanai et al., 2008). Thus, we only applied tSOS when subjects were in non-REM sleep Thymidylate synthase and cortical circuits preferentially resonate in the slow oscillation frequency, which ensured that the effect of tSOS expressed itself mainly as an enhanced SWA. Whereas, during the acute periods of stimulation, endogenous SWA generated in cortical tissue cannot be readily separated from activity in the same frequency band that is related to the stimulation signal, analysis of 1-min periods following the 4-min periods of tSOS confirmed

a distinct increase in SWA, especially during the first periods of stimulation. This observation agrees with previous studies (Marshall et al., 2006) in which a similar stimulation protocol conducted during nocturnal sleep enhanced both SWA and SWS during the stimulation-free intervals immediately after the periods of stimulation, with the effects being also most pronounced during the first three post-stimulation periods. Considering that, in those previous studies, owing to the strong contamination originating from the stimulation signal, EEG data during actual electrical stimulation could not be analysed, the present study including such analyses of EEG activity during ongoing stimulation represents a clear advance over this previous work.