5 ml) within 24 hours of birth and completed the hepatitis B vaccination according to the 0-1-2-11 month schedule. Immunoprophylaxis failure was defined as presence of HBsAg in blood of vaccinated children at 12 month of age. Results: At 12 month

of age, HBsAg was detected in blood of 17/246 vaccinated children (6.9%; infants born to mothers with HBsAg(+)/HBeAg(+) were likely 12 times higher to be infected by HBV than those born to mothers with HBsAg(+)/HBeAg(−)[OR (95% CI): 12 (3.3–43.2); infants with HBsAg and those with HBeAg in cord blood were likely 14.5 and 7.9 times to be infected by HBV than those without HBsAg and those without HBeAg in cordon blood, [OR (95% CI): 14.5 (1.9–111.4), and 7.9 (2.8–22.4); respectively]. Conclusion: The rate of HBsAg(+) in vaccinated check details children at 12 months was 6.9%; factors associated with immunoprophylaxis failure were presence of HBeAg in maternal blood at labour moment and presence of HBsAg and HBeAg in cord blood at birth. Key Word(s): 1. HBV markers; 2. HBV vaccination; 3. Prophylaxis failure; Presenting Author: MIN GAO MIN Corresponding Author: MIN GAO MIN Affiliations:

RXDX-106 in vitro Tianjin Second People’s Hospital Objective: To explore the relationship between liver tissues expression of HBcAg, subcellular localization of HBcAg with serum HBeAg expression, level of HBV replication and histologic activity. Methods: We enrolled 371 patients with chronic hepatitis B virus infection who underwent liver biopsy

at Tianjin Infectious disease Specialty Hospital between 2008–2010. The levels of alanine aminotransferase and HBV DNA level were simultaneously measured. MCE Compared the positive rate of serum HBeAg, the level of HBV replication., histologic activity in the patients with negative expression of HBcAg and nHBcAg, cHBcAg, n-cHBcAg expression. At the same time, observed the difference of the expression of HBcAg at different age-stage in HBeAg- positive patients and HBeAg- negative patients. Results: HBeAg was seropositive in 33.1% of patients with negative expression of HBcAg, 68.7% in those with nHBcAg, 62.3% in those with cHBcAg, and 84.5% in those with n-cHBcAg (p < 0.01). The percentage of patients with G ≥ 2 was lower (21.5% vs. 34.3%, 67.7%, 69.1%, p < 0.01) for the negative expression of HBcAg (cHBcAg) than for the nHBcAg, cHBcAg c-nHBcAg expression. Among them, the percentage of patients with G ≥ 2 for the cHBcAg and c-nHBcAg expression is higher than the nHBcAg expression (p < 0.01). The serum HBV DNA level was higher in nHBcAg among the groups. Among the cases in which serum HBeAg positive group, the expression rate for nHBcAg was 61.5% in ≤20 age stage while it was 11.5% in 20–39 age stage, 12.3% in ≥40 age stage. There were obvious increase about the percentage of patients with cHBcAg c-nHBcAg expression following the age increase (23.1%/7.7%; 26.4%/30.8%; 28.4%/45.4%), there was a statistical significance in the difference (X2 = 53.74, P < 0.01).