MtrB homologs with an N-terminal CXXC motif may thus represent a molecular signature unique to metal-reducing members of the Gammaproteobacteria. Dissimilatory metal-reducing bacteria occupy a central position in a variety of environmentally important processes, Selleckchem Hydroxychloroquine including the biogeochemical cycling of carbon and metals, the bioremediation of radionuclides and organohalides, and the generation of electricity in microbial fuel cells (Lovley & Coates, 1997; Thamdrup, 2000; Lovley et al., 2004; Logan, 2009).

Metal-reducing bacteria are scattered and deeply rooted throughout both prokaryotic domains (Lonergan et al., 1996; Vargas et al., 1998). Functional genes required for microbial metal

reduction display high sequence divergence, which limits their use as molecular biomarkers to examine fundamental ecological principles and environmental parameters controlling metal reduction in both natural and engineered systems. A variety of c-type cytochromes, for example, are key components of the electron transport systems of many metal-reducing bacteria (Weber et al., 2006; Richter et al., 2012), yet their widespread occurrence in nonmetal-reducing bacteria and high sequence divergence limit their utility as molecular biomarkers for tracking the presence and activity of metal-reducing bacteria as a functional group. The gene encoding the eukaryotic-like citrate synthase FDA approved Drug Library order (gltA) in the Geobacteraceae family has received attention as a molecular biomarker for

tracking the presence and activity of metal-reducing Geobacteraceae in subsurface environments (Bond et al., 2005; Wilkins et al., 2011). However, gltA is found only in members of the Geobacteraceae family, thus limiting its application as a molecular biomarker for metal-reducing bacteria outside the Geobacteraceae family. The large γ-proteobacteria class within the phylum Proteobacteria (Williams et al., 2010) Avelestat (AZD9668) was selected as a bacterial group to search for molecular signatures unique to metal-reducing bacteria outside the Geobacteraceae family. The large number of genera (over 250) and complete or nearly complete genomes (over 200) in the γ-proteobacteria class (Williams et al., 2010) facilitates nucleotide sequence comparisons of genes in both metal- and nonmetal-reducing bacteria, potentially aiding in the identification of molecular signatures unique to metal-reducing γ-proteobacteria. The γ-proteobacteria class includes Shewanella oneidensis, a gram-negative, facultative anaerobe that reduces a wide range of metals, including Fe(III) and Mn(IV) as terminal electron acceptor (Myers & Nealson, 1988; Venkateswaran et al., 1999).