The membrane was washed with TBST Histone Methyltransferase inhibitor buffer three times and then incubated with alkaline-phosphatase conjugated anti-mouse-IgG (1:2500, Sigma-Aldrich). The His6-tagged-protein band was visualized with 5-bromo-4-chloro-3-indolyl phosphate and nitro blue tetrazolium (Sigma-Aldrich) solution. Preparation

of M. smegmatisPG M. smegmatis PG was prepared from cell wall Selleckchem VX770 fractions as described previously [16–18]. Briefly, a 500 ml culture of M. smegmatis mc2155 in M9 minimal glucose medium was harvested when the OD600 reached 0.6, after which the cells were washed three times with pre-cooled phosphate buffered saline (PBS: 137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 2 mM KH2PO4, pH 7.0). The pellets were resuspended in distilled water to 0.2 g/ml, mixed with an equal volume of boiling 8% SDS added drop-wise

with continuous boiling for 30 min. A cell-wall-enriched fraction was obtained by centrifugation at 100,000 × g at 20°C for 60 min, followed by three washes with pre-cooled PBS. The pellet was washed with distilled water at least six times to remove the SDS. The sample was resuspended in 5 ml of buffer (10 mM Tris-HCl and 10 mM NaCl, pH 7.0) and then sonicated for 5 min. α-amylase and imidazole were added to the sample at final concentrations of Transmembrane Transporters inhibitor 100 μg/ml and 0.32 M, respectively, and the solution was incubated at 37°C for 2 h to remove glycogen. Afterwards, proteinase K was added to the sample at a final concentration of 100 μg/ml, followed by incubation at 37°C for 1.5 h to remove lipoprotein. The proteinase K solution was then inactivated by addition of an equal volume of boiling 8% SDS with vigorous stirring for 15 min. The mixture was ultracentrifuged at 100,000 × g at 20°C for 30 min. The pelleted material was washed as described above. The resulting mAGP (mycolyl-arabinogalactan-peptidoglycan) complex was washed with acetone and dried under a vacuum. Mycolic

acids were removed with 1% potassium hydroxide in methanol at 37°C for 72 h. After room temperature centrifugation at 27,000 × g for 30 min, the pelleted arabinogalactan-PG Phospholipase D1 was washed with distilled water twice and dried under a vacuum. Arabinogalactan was removed by washing with 49% hydrofluoridic acid at 4°C for 120 h with stirring. The resulting PG was pelleted by room temperature centrifugation at 27,000 × g for 30 min and then washed as described above. The PG was dissolved in 50 mM HEPS buffer (pH 7.0) at 1 mg/ml until further use. Deacetylase activity assays The acetyl group released from the PG was measured using an acetic acid detection kit (Roche, Darmstadt, Germany). Briefly, Rv1096 protein (2.88 μg/ml) prepared from ER2566/Rv1096 and M. smegmatis/Rv1096 were separately incubated with M. smegmatis PG. The reactions were performed at 37°C for 30 min and stopped by 10 min boiling.